DNA Investigation in Year 13 Biology Practical
Year 13 Biology students took part in an advanced practical investigating their own DNA through PCR and gel electrophoresis.
In this hands-on lesson, students began by extracting DNA from their own cells before using a polymerase chain reaction (PCR) machine to amplify a specific gene. This piece of cutting-edge equipment, not available in all schools, allowed students to explore real-world genetic analysis techniques commonly used in research and forensic science.
The amplified DNA was then analysed using gel electrophoresis, creating the distinctive “DNA ladder” seen in forensic investigations. Under UV light, students were able to visualise bands of DNA and determine which version of a gene they carry for tasting bitterness, a trait that influences how individuals perceive foods such as broccoli and coffee.
Handling professional laboratory equipment such as micropipettes and observing results emerge through UV visualisation gave students valuable insight into the techniques used by scientists beyond the classroom. The practical not only reinforced key concepts from the curriculum, but also highlighted the exciting opportunities available to students studying Biology.
